Journal: Histopathology

Article Title: Diagnostic features in paediatric MDS ‐ EB with UBTF ‐internal tandem duplication: defining a unique subgroup

doi: 10.1111/his.15378

Figure Lengend Snippet: Immunohistochemistry and special stain of UBFT ‐TD bone marrow biopsy. ( A ) Naphthol‐Chloracetatesterase stain shows a reduced granulopoeisis (red). ( B ) Erythropoiesis is atypically located, with expanded erythroid islets and an increased presence of immature cell forms (E‐cadherin immunohistochemistry). ( C ) Megakaryopoiesis is hyperplastic with small groups of hypolobulated cell forms and micromegakaryocytes (CD42b immunohistochemistry). Myeloblasts are negative for CD34 ( D ; CD34 immunohistochemistry) and positive for CD33 ( E ; CD33 immunohistochemistry). MPO‐positive myeloid precursors show prominent UBTF‐positive nucleoli ( F ; UBTF and MPO double‐immunofluorescence). Dispersed CD3‐positive T‐lymphocytes and CD20‐positive B‐lymphocytes are visible ( G ; CD3 immunohistochemistry and H ; CD20 immunohistochemistry). Small aggregates of CD123‐positive plasmocytoid dendritic cells are shown ( I ; CD123 immunohistochemistry). EWOG ID number D1000. [Color figure can be viewed at wileyonlinelibrary.com ]

Article Snippet: Immunostaining was performed for CD42b (SP219; Abcam, Cambridge, UK), E‐cadherin (EP700y; Zytomed Systems, Berlin, Germany), MPO (Agilent, Santa Clara, CA, USA), TdT (SEN28; Leica, Deer Park, IL, USA), CD20 (L26; Agilent), CD3(SP7; Zytomed Systems), CD123 (AbcamK), CD34 (QBEnd/10; Cell Marque, Rocklin, CA, USA) using an autostainer (Benchmark Ultra; Roche Diagnostics, Germany) following the manufacturer's instructions.

Techniques: Immunohistochemistry, Staining, Immunofluorescence

Journal: International Journal of Cancer

Article Title: FHL2 expression by cancer‐associated fibroblasts promotes metastasis and angiogenesis in lung adenocarcinoma

doi: 10.1002/ijc.35174

Figure Lengend Snippet: FHL2 KO in CAFs reduces CAF‐induced tube formation of MS1 cells and relationship of microvascular density and stromal FHL2 expression in clinical tumor samples. (A, B) Results of an endothelial cell tube formation assay. Murine endothelial MS1 cells were seeded in conditioned medium from CAF094 YFPhTERT cells (WT) or CAF094 YFPhTERT FHL2KO cells (KO). Photographs taken 6 h post‐seeding were analyzed. (A) Representative photographs of WT and KO groups are shown. (B) results of the quantification of tube formation are shown. Data represent the mean ± SEM. (C) Immunohistochemical analysis of CD34 in lung adenocarcinoma tissues from patients who underwent surgery. Scale bar, 50 μm. (Left) Representative image showing a tumor with high microvascular density. (Right) Representative image showing a tumor with low microvascular density. (D) Relationship of microvascular density and FHL2 expression. (Left) Relationship of microvascular density and tumoral FHL2 expression. (Right) Relationship of microvascular density and stromal FHL2 expression. **p < .01; *p < .05.

Article Snippet: The sections were then incubated at 4°C overnight with rabbit polyclonal anti‐human FHL2 antibody (1/200) (HPA006028, Atlas antibodies, Stockholm, Sweden) or mouse monoclonal anti‐human CD34 (1/400, clone QBEnd/10, NBP2‐32932, Novus biologicals, Novus Biological, Littleton, CO, USA).

Techniques: Expressing, Endothelial Cell Tube Formation Assay, Immunohistochemical staining